TLC Image Annotation Tool

Thin Layer Chromatography Analysis • Laboratory Management System

📁 Image Source
📸 Original Image
Annotation Tools
📋 Instructions
info TLC Analysis Instructions

Use the workflow below to annotate your TLC plate and calculate RF values accurately.

📋 Recommended Workflow:
  1. Load Your Image:
    • Select From ELN to use the image already linked to the experiment
    • Select Upload Local if you want to analyze a file from your computer
    • Confirm the TLC plate is clearly visible in the Original Image panel before you begin
  2. Set the Baseline and Solvent Front:
    • Click Min Line and draw it across the baseline / origin near the bottom of the plate
    • Click Max Line and draw it across the solvent front near the top of the plate
    • You can also try Auto Detect Lines first, then adjust manually if needed
  3. Add or Detect Spots:
    • Click Add Spots and select the center of each visible spot
    • Each spot is marked with an orange circle for easy review
    • Use Auto Detect Spots when the image is clean and the spot contrast is strong
  4. Organize the Plate (Optional):
    • Use V-Line to separate sample lanes
    • Use H-Line to add extra horizontal references
    • Enter labels when prompted so the RF table is easier to interpret
  5. Calculate RF Values:
    • Click Calculate RF after your lines and spots are in place
    • The system uses RF = distance traveled by spot / distance traveled by solvent front
    • Results appear in the table below with mixture labels, substance labels, RF values, and status indicators
  6. Save or Export Results:
    • 💾 Download CSV: Export the RF results table
    • 🖼️ Save Image: Store the annotated TLC image
    • ☁️ Upload to ELN: Send the calculated data back to the ELN record
💡 Pro Tips:
  • Start with the lines: Set the Min and Max lines before trusting RF calculations
  • Review auto-detection: Auto-detected spots and lines should be checked before saving results
  • Use lane labels: Labeled V-lines make multi-lane plates easier to read in the results table
  • Clean up mistakes: Remove incorrect annotations with the red ❌ marker when needed
  • Reset if necessary: Use Reset to clear the canvas and start over
⚠️ Important Notes:
  • Image quality matters: Use a clear, well-lit TLC image for the best detection results
  • Reference lines define the calculation: Min line = origin, Max line = solvent front
  • Spot placement matters: Click the center of each spot for the most accurate RF values
  • Validate the output: RF values should generally fall between 0 and 1
📊 RF Values Analysis Results
Mixture Label (X axis) Substance Label (Y axis) RF Value Status