TLC Image Annotation Tool

Thin Layer Chromatography Analysis • Laboratory Management System

📁 Image Source
📸 Original Image
Annotation Tools
📋 Instructions
info TLC Analysis Instructions

Follow these steps to analyze your TLC plate:

📋 Step-by-Step Guide:
  1. Image Ready: Your TLC plate image has been automatically loaded from the ELN system and appears in the left panel. You can now begin annotation.
  2. Set Reference Lines First:
    • Click Min Line → Draw a horizontal line at the solvent front (bottom baseline)
    • Click Max Line → Draw a horizontal line at the solvent front (top line where solvent reached)
  3. Mark Your Spots:
    • Click Add Spots button
    • Click directly on each spot in your TLC plate
    • Each spot will be marked with an orange circle
    • Add as many spots as needed for your analysis
  4. Add Lane Separators (Optional):
    • V-Line: Vertical lines to separate different sample lanes
    • H-Line: Additional horizontal reference lines
    • Type labels when prompted (e.g., "Sample A", "Standard", etc.)
  5. Calculate RF Values:
    • Click Calculate RF button
    • RF values will be automatically calculated using the formula: RF = Distance traveled by spot / Distance traveled by solvent
    • Results appear in the table below with spot coordinates and RF values
  6. Export Your Results:
    • 💾 Download CSV: Save RF data as spreadsheet
    • 🖼️ Save Image: Download annotated TLC image
    • ☁️ Upload to ELN: Send results to laboratory database
💡 Pro Tips:
  • Accuracy: Zoom in on your image for precise spot marking
  • Order Matters: Always set Min/Max lines before adding spots
  • Multiple Lanes: Use V-Lines to organize different samples
  • Corrections: Click the red ❌ on any annotation to delete it
  • Reset: Use the Reset button to start over if needed
⚠️ Important Notes:
  • Image Quality: Use clear, well-lit TLC plate photos
  • Reference Lines: Min line = starting point, Max line = solvent front
  • Spot Center: Click the center of each spot for accurate measurements
  • Data Validation: Review RF values - they should be between 0 and 1
📊 RF Values Analysis Results
Mixture Label (X axis) Substance Label (Y axis) RF Value Status